Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
Host
Rabbit
Immunogen
The antibody was produced against synthesized phosphopeptide derived from human HDAC4/HDAC5/HDAC9 around the phosphorylation site of serine 246/259/220 (T-A-SP-EP).
Isotype
IgG
Quantity
100 µg
Reactivity
Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
100µg/100ul
Description
Specificity: HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody detects endogenous levels of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC
Description
WB: 1:500~1:1000 IHC:1:50~1:100, WB ; IHC
Dilution
WB
1:500-1:1000~
IHC
1:50-1:100~
Reviews of Anti-HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody