The BUB family of serine/threonine kinases is a major target of oncology therapeutic research and development. hBUB1 and hBUBR1 are kinases that associate with hBUB3 and are essential components of the mitotic checkpoint. hBUBR1 appears to provide two checkpoint functions by monitoring CENP-E-dependent activities at the kinetochore and by regulating cyclosome/APC activity.hBUBR1 is required in order for microtubule inhibitors to arrest cells in mitosis. Non-mutational inactivation of hBUB1 and hBUBR1 appear related to stage of colon cancer and could be related to aneuploidy in human colorectal and other cancers.
Host
Mouse
Isotype
IgG1
Quantity
100 µg
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100µg/100ul
Format
Liquid
Purity
Purified ascites
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IF
Description
Titrate for optimal dilution for your application., WB ; IP ; IF