Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro.
Host
Mouse
Immunogen
A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
Isotype
IgG3
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: HA-tag monoclonal antibody. Recognizes HA-tagged tagged proteins overpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IF, IHC, ICC
Description
ICC/IHC/IF/IP 1:200 dilution. WB 1:1000 dilution., WB ; IP ; IF ; IHC ; ICC
Dilution
IP
1:200
WB
1:1000
IF
1:200
IHC
1:200
ICC
1:200
Reference
Armistead, J et al. "Mutation of a Gene Essential for Ribosome Biogenesis, EMG1, Causes Bowen-Conradi Syndrome." Am J Hum Genet 84 (6):728-739 (2009). DOI: 10.1016/j.ajhg.2009.04.017. Application: Immunoblot.
Barr, DJ et al. "Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells." J Cell Sci 121:3155-66 (2008). PubMed: 18765569.
Bronnimann, MP et al. "A transmembrane domain and GxxxG motifs within L2 are essential for papillomavirus infection." J Virol 87 (1):464-473 (2013). DOI: 10.1128/JVI.01539-12. Application: Western Blot.
Burston, HE et al. "Regulators of yeast endocytosis identified by systematic quantitative analysis." J. Cell Biol. 185:1097-110 (2009). PubMed: 19506040.
Byongsun, L et al. "Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2:303-313 (2017). DOI: 10.1016/j.bbamcr.2016.11.013. Application: Loading Control Antibody.
Charbonneau, Me et al. "Autoprocessing of the Escherichia coli AIDA-I autotransporter: a new mechanism involving acidic residues in the junction region." J Biol Chem 284 (25):17340-17351 (2009). DOI: 10.1074/jbc.M109.010108. Application: Immunodection.
Cho, HJ et al. "EphrinB1 Interacts with CNK1 and Promotes Cell Migration through JNK Activation." J. Biol. Chem. : (2014). PubMed: 24825906.
Cho, HJ et al. "EphrinB1 promotes cancer cell migration and invasion through the interaction with RhoGDI1." Oncogene : (2017). DOI: 10.1038/onc.2017.386. PubMed: 29059157. Application: Western Blot.
Choi, JR et al. "p300-mediated acetylation increased the protein stability of HIPK2 and enhanced its tumor suppressor function." Sci. Rep. 1:16136 (2017). DOI: 10.1038/s41598-017-16489-w. Application: Western Blot.
Daniel, CJ et al. "Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation." Methods Mol Biol 1012:65-76 (2013). DOI: 10.1007/978-1-62703-429-6_5. PubMed: 24006058. Application: Immunoprecipitation.
Dupé, A et al. "An Alba-domain protein contributes to the stage-regulated stability of amastin transcripts in Leishmania." Mol. Microbiol. 91:548-61 (2014). PubMed: 24620725.
Dupé, A et al. "Differential Subcellular Localization of Leishmania Alba-Domain Proteins throughout the Parasite Development." PLoS One 9:e0137243 (2015). DOI: 10.1371/journal.pone.0137243. PubMed: 26334886. Application: Immunofluorescence.
Escamilla-Powers, JR et al. "The tumor suppressor protein HBP1 is a novel c-myc-binding protein that negatively regulates c-myc transcriptional activity.." J Biol Chem 285 (7):4847-4858 (2010). DOI: 10.1074/jbc.M109.074856. Application: Immunoprecipitation.
Gong, R et al. "Bispecific engineered antibody domains (nanoantibodies) that interact noncompetitively with an HIV-1 neutralizing epitope and FcRn." PLoS ONE 7:e42288 (2012). PubMed: 22879932.
Green, JL et al. "The C. elegans ROR receptor tyrosine kinase, CAM-1, non-autonomously inhibits the Wnt pathway." Development 124 (22):4053-4062 (2007). PubMed: 17942487. Application: Immunoblotting.
Harvey, MC et al. "Distinct effects of the mesenchymal dysplasia gene variant of murine Patched-1 protein on canonical and non-canonical Hedgehog signaling pathways." J. Biol. Chem. 289:10939-49 (2014). PubMed: 24570001.
Hayashi, A et al. "Pyripyropenes, fungal sesquiterpenes conjugated with α-pyrone and pyridine moieties, exhibits anti-angiogenic activity against human umbilical vein endothelial cells." Biol Pharm Bull 32 (7):1261-1205 (2009). PubMed: 19571395. Application: Western Blot.
Hwang, YS et al. "The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1." Genes Dev. 27:491-503 (2013). PubMed: 23475958.
Irie, T et al. "Inhibition of interferon regulatory factor 3 activation by paramyxovirus V protein." J. Virol. 86:7136-45 (2012). PubMed: 22532687.
Irie, T et al. "Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection." Virology 405 (2):334-341 (2013). DOI: 10.1016/j.virol.2010.06.031. PubMed: 20605035. Application: Western Blot.
Ji, YJ et al. "EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1." Nat Commun 5:3516 (2014). PubMed: 24662724.
Jin, H et al. "A Herpesvirus Virulence Factor Inhibits Dendritic Cell Maturation through Protein Phosphatase 1 and IκB Kinas." J Virol 85 (7):3397-3407 (2011). DOI: 10.1128/JVI.02373-10. Application: Western Blot.
Kang, M et al. "Human leucine zipper protein promotes hepatic steatosis via induction of apolipoprotein A-IV." FASEB J. 6:2548-2561 (2017). DOI: 10.1096/fj.201601227R. PubMed: 28246167. Application: Tag Antibody.
Lee, HK et al. "Drought stress-induced Rma1H1, a RING membrane-anchor E3 ubiquitin ligase homolog, regulates aquaporin levels via ubiquitination in transgenic Arabidopsis plants." Plant Cell 21:622-41 (2009). PubMed: 19234086.
Lee, HS et al. "EphrinB1 controls cell-cell junctions through the Par polarity complex." Nat. Cell Biol. 10:979-86 (2008). PubMed: 18604196.
Lee, JY et al. "Cell growth defect factor1, chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis." Plant Cell 25:3944-60 (2013). PubMed: 24151298.
Leung, GP et al. "Rtt107 is required for recruitment of the SMC5/6 complex to DNA double strand breaks." J Biol Chem 286 (29):26250-7 (2011). PubMed: 21642432. Application: Immunoprecipitation.
Li, P et al. "Multiple roles of the candidate oncogene ZNF217 in ovarian epithelial neoplastic progression." Int J Cancer 120 (9):1863-1873 (2007). PubMed: 17266044. Application: Western Blot.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors." Proteomics 12:2094-106 (2012). PubMed: 22623184.
Ma, Y et al. "Inhibition of TANK binding kinase 1 by herpes simplex virus 1 facilitates productive infection." J. Virol. 86:2188-96 (2012). PubMed: 22171259. Application: Immunoblot.
Morgan, CP et al. "TRPV5, TRPV6, TRPM6, and TRPM7 do not contribute to hair-cell mechanotransduction." bioRxiv :204172 (2017). DOI: 10.1101/204172. Application: Western Blot.
Neilson, KM et al. "Specific domains of FoxD4, 5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev. Biol. 365:363-75 (2012). PubMed: 22425621.
Parker, JG et al. " The Contribution of NMDA Receptor Signaling in the Corticobasal Ganglia Reward Network to Appetitive Pavlovian Learning." J Neurosci 31 (31):11362-11369 (2011). DOI: 10.1523/JNEUROSCI.2411-11.2011. Application: Immunostaining.
Prieto-Echagüe, V et al. "Regulation of Ack1 localization and activity by the amino-terminal SAM domain." BMC Biochem. 11:42 (2010). PubMed: 20979614.
Son, J et al. "Pick1 modulates ephrinB1-induced junctional disassembly through an association with ephrinB3." Biochem. Biophys. Res. Commun. 1:659-667 (2014). PubMed: 24937449.
Su, B et al. "Adhesion-mediated cytoskeletal remodeling is controlled by the direct scaffolding of Src from FAK complexes to lipid rafts by SSeCKS, AKAP12." Oncogene 32:2016-26 (2013). PubMed: 22710722.
Verpooten, D et al. "Control of TANK-binding kinase 1-mediated signlaing by the y134. 5 protein of herpes simplex virus 1.." J Biol Chem 284 (2):1097-1105 (2009). DOI: 10.1074/jbc.M805905200. Application: Immunoblot.
Whittington, , N. et al. "Sox21 regulates the progression of neuronal differentiation in a dose-dependent manner." Developmental Biology 397 (2):237-247 (2014). PubMed: 25448693.
Reviews of Anti-HA Tag Antibody (Mouse Monoclonal)