GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Host
Mouse
Immunogen
Anti-GST antibody has been raised against whole GST protein.
Isotype
IgG2a
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Detects overexpressed glutathione-transferase (GST) fusion proteins. Recognizes native and denatured forms of purified GST or GST fusion proteins
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, ELISA
Description
Western blot (1:1000), Immunoprecipitation and Immunostaining (1:200)., WB ; IP ; ELISA
Dilution
WB
1:1000
Ist
1:200
IP
1:200)
Reference
Ding, Y et al. "ATX1-generated H3K4me3 is required for efficient elongation of transcription, not initiation, at ATX1-regulated genes." PLoS Genet. 8:e1003111 (2012). PubMed: 23284292.
Ding, Y et al. "Two distinct roles of ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1) at promoters and within transcribed regions of ATX1-regulated genes." Plant Cell 23 (1):350-363 (2011). DOI: 10.1105/tpc.110.080150. Application: Immunoblot.
Gusarova, GA et al. "Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta." Mol Cell Biol 13:3455-3464 (2009). DOI: 10.1128/MCB.00054-09. Application: Western Blot.
Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1." Biochem Biophys Res Commun 393 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. Application: Immunoblot.
Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
Xue, ZH et al. "A role of kindlin-3 in integrin αMβ2 outside-in signaling and the Syk-Vav1-Rac1/Cdc42 signaling axis." PLoS One 8 (2):356911 (2013). DOI: 10.1371/journal.pone.0056911. Application: Reporter of siRNA transduction system.
Reviews of Anti-GST Tag Antibody (Mouse Monoclonal)