Synthetic peptide derived from Human Growth Hormone.
Isotype
IgG
Quantity
100 µl
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100µg/100ul
Description
Specificity: This Polyclonal antibody is directed against human Growth Hormone protein. The product was purified from serum by protein A chromatography. Expect reactivity with human Growth Hormone. Cross-reactivity with Growth Hormone from other sources has not been determined.Relevance: Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC
Description
Recommended dilutionsWB: 1/500~5000.Predicted molecular weight: 22~24 kDa.IHC: This antibody can be used on paraffin embedded tissue after enzymatic antigen retrieval.Dilute approximately 1/50~200.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB ; IHC