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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GPR68 (OGR1) (extracellular) Antibody

SKU : BHA21300453

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Alomone Labs

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Mfr.No.	AGR-042, AGR-042-CF_0.2 ml, AGR-042_0.2 ml, AGR-042_25 mcl, AGR-042_50 mcl
Lead Time	1-2 Business Days
Immunogen Species	Mouse
Background	All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.
Clonality	Polyclonal
Description	Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.
Formulation	PBS pH7.4
Host	Rabbit
Immunogen Region	2nd extracellular loop
Isotype	Rabbit IgG
Reactivity	Human, Rat, Mouse
Reconstitution	0.2 ml double distilled water (DDW).
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
UNSPSC Code	41116161
Note	Homology: Rat, mouse, human - 14/15 amino acid residues identical Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Negative Control: BLP-GR042 Specifictiy: GPR68 Is Toxin: No Standard quality control of each lot: Western blot analysis Antigen preadsorption control: 1 ug peptide per 1 ug antibody

Accession Number	Q8BFQ3
Gene Id	238377
Target	Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor
Sequence	(C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Conjugates	Unconjugated

Concentration	1 mg/ml
Form	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Preservative	No Preservative
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	IFC, LCI, WB
Dilution	Immunohistochemistry paraffin embedded sections ihc: Contact Biohippo for information Western blot wb: 1:200

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300453-1	AGR-042-CF_0.2 ml	0.2 ml(Carrier Free)	$994		Add to Cart
BHA21300453-2	AGR-042_0.2 ml	0.2 ml	$795		Add to Cart
BHA21300453-3	AGR-042_25 mcl	25 mcl	$597		Add to Cart
BHA21300453-4	AGR-042_50 mcl	50 mcl	$697		Add to Cart

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