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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GPR119 Antibody

SKU : BHA21300448

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Alomone Labs

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Mfr.No.	AGR-032, AGR-032-CF_0.2 ml, AGR-032_0.2 ml, AGR-032_25 mcl, AGR-032_50 mcl
Lead Time	1-2 Business Days
Immunogen Species	Rat
Background	Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Galphas coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1); this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .
Clonality	Polyclonal
Description	Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (#AGR-032) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.
Formulation	PBS pH7.4
Host	Rabbit
Immunogen Region	2nd intracellular loop
Isotype	Rabbit IgG
Reactivity	Rat, Mouse
Reconstitution	0.2 ml double distilled water (DDW).
Shipping	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Synonyms	Glucose-dependent insulinotropic receptor, GPCR2
UNSPSC Code	41116161
Note	Homology: Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry Negative Control: BLP-GR032 Specifictiy: GPR119 Is Toxin: No Standard quality control of each lot: Western blot analysis Antigen preadsorption control: 1 ug peptide per 1 ug antibody

Accession Number	Q7TQN8
Gene Id	302813
Target	Glucose-dependent insulinotropic receptor, GPCR2
Sequence	CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Conjugates	Unconjugated

Concentration	1 mg/ml
Form	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Preservative	No Preservative
Purity	Affinity purified on immobilized antigen.
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Applications	WB
Dilution	Immunohistochemistry paraffin embedded sections ihc: Contact Biohippo for information Western blot wb: 1:400

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA21300448-1	AGR-032-CF_0.2 ml	0.2 ml	$994		Add to Cart
BHA21300448-2	AGR-032_0.2 ml	0.2 ml	$795		Add to Cart
BHA21300448-3	AGR-032_25 mcl	25 mcl	$597		Add to Cart
BHA21300448-4	AGR-032_50 mcl	50 mcl	$697		Add to Cart

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