GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer.
Host
Rabbit
Immunogen
A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived , A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived
Quantity
100 µl
Reactivity
Rat, Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
1µg/ul
Description
Specificity: This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P).
Format
Liquid
Purity
Filter purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC
Description
This is an extremely high titre antibody that detects 500 ng of peptide antigen on western blotting up to a dilution of 1: 500000. A dilution o f 1: 1000 to 1: 5000 is recommended for immunohistochemistry depending on detection method but end user will need to determine dilution to suit application. Antigen retrieval is recommended. For Western blotting it is recommended that several dilutions be tested in the range 1: 5000 to 1: 50000 although higher dilutions could be use if the antigen is in abundance in sample. The optimal dilution should be determined by the end user., WB ; IHC
Dilution
WB
1:500000
WB
1:5000-1:50000
Reviews of Anti-Glutathione peroxidase 3 (GPx-P, 135-154) Antibody