Anti-glutathione peroxidase 1 (GPx-1, 175-194) Antibody

Mfr.No.	Y075104
Categories	Primary Antibodies
Clonality	polyclonal
Description	Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.
Host	sheep
Immunogen	A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Quantity	100 µl
Reactivity	Rat, Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.02% sodium azide.
Concentration	1µg/ul
Description	Specificity: This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein.
Format	Liquid
Purity	Filter purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, IHC

Description

This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. The optimal dilution should be determined by the end user. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes., WB ; IHC

Dilution

IHC	1:2000-1:20000
WB	1:10000-1:100000
ELISA	1:2000-1:20000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10903390	Y075104	100 ul	$802.50		Add to Cart

Anti-glutathione peroxidase 1 (GPx-1, 175-194) Antibody

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