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Home / Antibodies

Anti-Glutamate Dehydrogenase Antibody

SKU : BHA10920074

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y052277
Categories	Primary Antibodies
Clonality	polyclonal
Description	Glutamate is a majorexcitatory neurotransmitter. One enzyme central to themetabolism of glutamate is glutamate dehydrogenase(GDH1; EC 1.4.1.3), that catalyzes the reversibledeamination of L-glutamate to 2-oxoglutarate usingNAD+ or NADP+. Mammalian GDH is composed of sixidentical subunits, and the regulation of GDH is verycomplex. It has been a major goal to identify thesubstrate and regulatory binding sites of GDH. It is onlyin recent years that the three-dimensional structure ofGDH from microorganisms is available. Very recently,crystallization of bovine liver GDH was reported for thefirst time from the mammalian sources. However,remarkably little is known about the detailed structureof mammalian GDH, especially the brain enzymes.
Formulation	Liquid
Host	Rabbit(Rb)
Guarantee	abm guarantees that all our Anti-Glutamate Dehydrogenase Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full lengthGlutamate Dehydrogenase protein isolated from Bovine Liver.
Isotype	IgG
Quantity	100 μl
Recommended Dilution	This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivityshould be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within themitochondrial matrix. Expect a band approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by western blotting in the appropriate cell or tissue extract.
Shipping Condition	Ice Packs
Species Reactivity	Bovine
Specificity	This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay byimmunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [BovineLiver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 andGDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including greenmonkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken,mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivityagainst Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Gene Name	Glutamate Dehydrogenase
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C

Description	ELISA ; WB

Reference	Dawson, NJet al. "An enzymatic bridge between carbohydrate and amino acid metabolism: regulation of glutamate dehydrogenase by reversible phosphorylation in a severe hypoxia-tolerant crayfish." J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. 182:331-40 (2012).PubMed: 22076534.

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SKU	Supplier No.	Size	Your price	Quantity
BHA10920074	Y052277	100 ul	$1162.50		Add to Cart

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