Anti-GFP Tag Antibody (Mouse Monoclonal)

Mfr.No.	G096-1mg
Categories	Primary Antibodies ; Tag & Cell Markers
Clonality	monoclonal
Description	Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Formulation	Liquid
Host	Mouse(Mu)
Guarantee	abm guarantees that all our Anti-GFP Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa).
Isotype	IgG1
Quantity	1.0 mg
Recommended Dilution	WB 1:1000. For other applications, the researcher should determine the optimal working dilution.
Relevance	Has sensitivity of 1-10ng of purified GFP or GFP fusion protein (from a western blot developed with ECL).
Shipping Condition	Ice Packs
Specificity	Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.

Gene Name	GFP
Clone No	LGB-1

Concentration	100µg/100ul
Purity	Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C