Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Host
Mouse
Immunogen
GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa).
Isotype
IgG1
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.Relevance: Has sensitivity of 1-10ng of purified GFP or GFP fusion protein (from a western blot developed with ECL).
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, ELISA
Description
WB 1:1000. For other applications, the researcher should determine the optimal working dilution., WB ; ELISA
Dilution
WB
1:1000
Reference
Bae, S et al. "A minimum fragment of polyhedrin for higher expression of foreign proteins in a baculovirus expression system." J. Asia-Pac. Entomol. 2:591-597 (2017). DOI: 10.1016/j.aspen.2017.03.021. Application: Western Blot.
Choi, JR et al. "p300-mediated acetylation increased the protein stability of HIPK2 and enhanced its tumor suppressor function." Sci. Rep. 1:16136 (2017). DOI: 10.1038/s41598-017-16489-w. Application: Western Blot.
Kim, K et al. "Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)." Biochem Biophys Res Commun 4:857-862 (2015). PubMed: 26590017.
Moncivais, K et al. "Tetracycline repressor-based mammalian two-hybrid systems." Methods Mol Biol 812:259-273 (2012). DOI: 10.1007/978-1-61779-455-1_15. PubMed: 22218865. Application: Western Blot.
Vohnoutka, RB et al. "Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.." Biol Open 10:1516-1527 (2017). DOI: 10.1242/bio.028522. PubMed: 28882840. Application: Western Blot.
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No Description Available.
Reviews of Anti-GFP Tag Antibody (Mouse Monoclonal)