The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Isotype
IgG
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Recognize GFP and its variants.
Format
Liquid
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP, IF, IHC, ELISA
Description
ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200, WB ; IP ; IF ; IHC ; ELISA
Dilution
ELISA
1:40,000
WB
1:400-1:2,000
IHC
1:200-1:1,000
IF
1:1,000
IP
1:200
Reference
Luchniak, A et al. "Structure-function analysis of yeast." Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.
Sutton, LM et al. "Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease." Hum Mol Genet 22 (3):452-465 (2013). DOI: 10.1093/hmg/dds441. Application: Immunoprecipitation.
No Description Available.
Reviews of Anti-GFP Tag Antibody (Goat Polyclonal)