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Home / Primary Antibodies / Polyclonal Antibodies

Anti-GAD65/GAD2 Antibody Picoband®

SKU : BHA21006771

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Mfr.No.	PA1403, PA1403-10ug, PA1403-Biotin, PA1403-Cy3, PA1403-Dylight488, PA1403-Dylight550, PA1403-Dylight594, PA1403-FITC, PA1403-HRP, PA1403-APC, PA1403-PE, PA1403-iFluor647, PA1403-carrier-free
Alternative Names	Glutamate decarboxylase 2
Background	Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alteative splicing results in multiple transcript variants that encode the same protein.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins
Description	Boster Bio Anti-GAD65/GAD2 Antibody catalog # PA1403. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the production of GABA.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, identical to the related mouse and rat sequences.
Isotype	Rabbit IgG
Molecular Weight	65 kDa
Reactivity	Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity	No cross reactivity with other proteins.
Synonyms	Glutamate decarboxylase 2;4.1.1.15;65 kDa glutamic acid decarboxylase;GAD-65;Glutamate decarboxylase 65 kDa isoform;GAD2;GAD65;
Tissue Specificity	Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Uniprot	Q05329
Gene Name	GAD2
Sequence	Belongs to the group II decarboxylase family.
Subcellular Location	Cytoplasm, cytosol . Cytoplasmic vesicle . Cell junction, synapse, presynaptic cell membrane ; Lipid-anchor . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	IHC, WB
Description	Weste blot, 0.1-0.5ug/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Rat, By Heat<br>

Reference	1. "Entrez Gene: GAD2 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)".<br /><br />2. Meyre, D., Boutin, P., Tounian, A., Deweirder, M., Aout, M., Jouret, B., Heude, B., Weill, J., Tauber, M., Tounian, P., Froguel, P. Is glutamate decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children J. Clin. Endocr. Metab. 90: 2384-2390, 2005.<br /><br />3. Zhang, Z., Cai, Y. Q., Zou, F., Bie, B., Pan, Z. Z. Epigenetic suppression of GAD65 expression mediates persistent pain. Nature Med. 17: 1448-1455, 2011.<br /><br />

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21006771-1	PA1403	-	100 ug	$370		Add to Cart
BHA21006771-2	PA1403-10ug	Sample	10 ug	$99		Add to Cart
BHA21006771-3	PA1403-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21006771-4	PA1403-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21006771-5	PA1403-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21006771-6	PA1403-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21006771-7	PA1403-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21006771-8	PA1403-FITC	FITC	100 ug	$570		Add to Cart
BHA21006771-9	PA1403-HRP	HRP	100 ug	$570		Add to Cart
BHA21006771-10	PA1403-APC	APC	100 ug	$670		Add to Cart
BHA21006771-11	PA1403-PE	PE	100 ug	$670		Add to Cart
BHA21006771-12	PA1403-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21006771-13	PA1403-carrier-free	carrier-free	100 ug	$370		Add to Cart

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