Raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes.
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Description
Specificity: Cross reacts with fibrillarin in human, rat, plant, Drosophila, C. elegans and S. pombe.
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF, ICC
Description
For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250., WB ; IF ; ICC
Dilution
WB
1:1,000
D
1:500-1:2,500
WB
1:250
IF
1:500-1:2,500
IF
1:250
No Description Available.
Reviews of Anti-Fibrillarin (Mouse Monoclonal) Antibody