This antibody reacts with the extracellular domain of EGFR and blocks the EGF/TGF??induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin.
Host
Mouse
Immunogen
Microsomes from A431 cells.
Isotype
IgG2a Kappa
Positive Control
A431 cells, Breast or Bladder cancer
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P00533
Uniprot
488293
Gene Id
1956
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
IP, FACS
Description
Affinity Purification (For coupling, order Ab without BSA); Blocks Ligand-induced Activation of EGFR; Inhibits Proliferation of A431 Cells (Order Ab without sodium azide); Arrests Tumor Growth in vivo (Order Ab without BSA &Azide); Optimal dilution for a specific application should be determined., IP ; FACS
Dilution
IP
1-2 �g/500ug protein Lysate
FACS
0.5-1μg/million cells in 0.1ml
Reviews of Anti-EGFR (Epidermal Growth Factor Receptor) Antibody BHA10200882