JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Primary Antibodies / Polyclonal Antibodies

Anti-DFFA/ICAD Antibody Picoband®

SKU : BHA21003041

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Boster

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	A03671-4, A03671-4-10ug, A03671-4-Biotin, A03671-4-Cy3, A03671-4-Dylight488, A03671-4-Dylight550, A03671-4-Dylight594, A03671-4-FITC, A03671-4-HRP, A03671-4-APC, A03671-4-PE, A03671-4-iFluor647, A03671-4-carrier-free
Alternative Names	acetyl-CoA carboxylase beta
Background	DNA fragmentation factor subunit alpha (DFFA), also known as Inhibitor of caspase-activated DNase (ICAD), is a protein that in humans is encoded by the DFFA gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alteatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-DFFA/ICAD Antibody Picoband® catalog # A03671-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (PubMed:20457939, PubMed:19236960, PubMed:20952656). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (PubMed:20457939, PubMed:1
Host	Rabbit
Immunogen Region	E.coli-derived human DFFA/ICAD recombinant protein (Position: E14-S308).
Isotype	Rabbit IgG
Molecular Weight	45 kDa
Reactivity	Human, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Acetyl-CoA carboxylase 2; ACC-beta; Biotin carboxylase; ACACB; ACC2, ACCB
Tissue Specificity	Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Uniprot	O00273
Gene Id	1676
Gene Name	DFFA
Subcellular Location	Nucleus. Cytosol.

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	WB, Flow Cytometry, ELISA
Description	Weste blot, 0.25-0.5ug/ml, Human, Rat<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Abel, F., Sjoberg, R.-M., Ejeskar, K., Krona, C., Martinsson, T. Analyses of apoptotic regulators CASP9 and DFFA at 1p36.2, reveal rare allele variants in human neuroblastoma tumours. Brit. J. Cancer 86: 596-604, 2002.<br /><br />2. Inohara, N., Koseki, T., Chen, S., Wu, X., Nunez, G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 17: 2526-2533, 1998.<br /><br />3. Leek, J. P., Carr, I. M., Bell, S. M., Markham, A. F., Lench, N. J. Assignment of the DNA fragmentation factor gene (DFFA) to human chromosome bands 1p36.3-p36.2 by in situ hybridization. Cytogenet. Cell Genet. 79: 212-213, 1997.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21003041-1	A03671-4	-	100 ug	$370		Add to Cart
BHA21003041-2	A03671-4-10ug	Sample	10 ug	$99		Add to Cart
BHA21003041-3	A03671-4-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21003041-4	A03671-4-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21003041-5	A03671-4-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21003041-6	A03671-4-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21003041-7	A03671-4-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21003041-8	A03671-4-FITC	FITC	100 ug	$570		Add to Cart
BHA21003041-9	A03671-4-HRP	HRP	100 ug	$570		Add to Cart
BHA21003041-10	A03671-4-APC	APC	100 ug	$670		Add to Cart
BHA21003041-11	A03671-4-PE	PE	100 ug	$670		Add to Cart
BHA21003041-12	A03671-4-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21003041-13	A03671-4-carrier-free	carrier-free	100 ug	$370		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.