Specificity: Anti-D tag Antibody recognises over-expressed proteins containing D epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP
Description
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000. Immunoprecipitation amd immunostaining at dilutions of 1:200., WB ; IP
Dilution
WB
1:1000
Ist
1:200
IP
1:200
Reference
Jo, SH et al. "Txnip contributes to impaired glucose tolerance by upregulating the expression of genes involved in hepatic gluconeogenesis in mice." Diabetologia 56 (12):2723-2732 (2013). DOI: 10.1007/s00125-013-3050-6. Application: Western Blot.
Lu, J. "JWA inhibits melanoma angiogenesis by suppressing ILK signaling and is an independent prognostic biomarker for melanoma." Carcinogenesis 34:2778-88 (2013). PubMed: 24064223.
Rothenburg, S et al. "Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: varying the number of double-stranded RNA binding domains and lineage-specific duplications." BMC Biol. 6:12 (2008). PubMed: 18312693. Application: Immunoblot.
Tran, D et al. "A mutation in VAPB that causes amyotrophic lateral sclerosis also causes a nuclear envelope defect." J Cell Sci 125:2831-6 (2012). PubMed: 22454507.
No Description Available.
Reviews of Anti-D Tag (Rabbit Polyclonal) Antibody