Anti-Cytokeratin, Acidic (Type I or LMW) Antibody BHA10207684

Mfr.No.	MSM4X-253-P1ABX
Alternative Names	K1B ; KRT1B ; Keratin, type II cytoskeletal 1b ; K77 ; CK-1B ; Keratin 1B ; Keratin-77 ; Cytokeratin-1B ; Type-II Keratin Kb39
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	17q21.2
Clonality	Monoclonal
Description	This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50ā€™, 48, 46, 45, and 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Host	Mouse
Immunogen	Human epidermal keratin
Positive Control	Skin, Squamous cell carcinoma (SCC)
Reactivity	Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken, Turtle
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q7Z794
Uniprot	334989
Gene Id	374454

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
WB	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207684	MSM4X-253-P1ABX	100µg	$393.20		Add to Cart

Anti-Cytokeratin, Acidic (Type I or LMW) Antibody BHA10207684

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