The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R).
Quantity
100 µg
Reactivity
Rat, Mouse, Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1µg/ul
Description
Specificity: CREB (phospho-Ser133) antibody detects endogenous levels of CREB only when phosphorylated at serine 133.
Format
Liquid
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IHC
Description
WB: 1:500~1:1000 IHC: 1:50~1:100, WB ; IHC
Dilution
WB
1:500-1:1000~
IHC
1:50-1:100~
Reference
Mitsui, S et al. "A mental retardation gene, motopsin/neurotrypsin/prss12, modulates hippocampal function and social interaction." Eur J Neurosci 30(12):2368-78 (2009). DOI: 10.1111/j.1460-9568.2009.07029.x. PubMed: 20092579.
Tsuruta, H et al. "Megadose vitamin C suppresses sulfoconjugation in human colon carcinoma cell line Caco-2." Toxicol In Vitro 25:500-4 (2011). PubMed: 21144891.