Intracytoplasmic vacuoles of endothelial cells(secreted)
Chromosome Location
6p21.3
Clonality
monoclonal
Description
This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.ĀC4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
Host
Mouse
Immunogen
Recombinant human Complement 4d protein
Positive Control
Rejected Renal Transplant Tissue
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P0C0L4; P0C0L5
Uniprot
534847; 720022
Gene Id
720;721
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., ELISA ; IF ; IHC-F
Dilution
ELISA
For coating use Ab at 1-2μg/ml,order Ab without BSA
IF
1:50-1:100
IHC-F
1:200-1:400 for 30 minutes at RT
Reviews of Anti-Complement 4d (C4d) Antibody BHA10205515