Anti-Clathrin (619-638) Antibody

Mfr.No.	Y075069
Categories	Primary Antibodies
Clonality	polyclonal
Description	Clathrin is a major protein component of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice to either the plasma membrane or the trans-Golgi network. These specialised organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Clathrin localises to the cytoplasmic face of coated pit and vesicle membranes.
Host	Rabbit
Immunogen	A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in m, A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in m
Quantity	100 µl
Reactivity	Rat
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.02% sodium azide.
Concentration	1µg/ul
Description	Specificity: This antibody has been shown to be specific for clathrin.
Format	Liquid
Purity	Filter purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, IHC

Description

This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy ie a dilution range of 1: 100 to 1: 400. For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended., WB ; IHC

Dilution

IHC	1:100-1:4000
WB	1:1000-1:8000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10902345	Y075069	100 ul	$802.50		Add to Cart

Anti-Clathrin (619-638) Antibody

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