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Home / Antibodies

Anti-CD44 Antibody

SKU : BHA10922465

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y059133
Categories	Primary Antibodies
Clonality	monoclonal
Description	CD44 is a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Formulation	Liquid
Host	Mouse(Mu)
Guarantee	abm guarantees that all our Anti-CD44 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	COS-7 cells (African Green Monkey).
Isotype	IgG1
Quantity	100 μg
Recommended Dilution	Western Blotting recommended dilution: 1:500Positive control:Kg-1a human acute leukemia cell lysateJURKAT human leukemia T-cell lysateSample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.60 min on vertical incubatorApplication note: Non-reducing conditions. SDS-PAGE (6% separating gel).
Shipping Condition	Ice Packs
Species Reactivity	Human
Specificity	The antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1)

Gene Name	CD44
Clone No	Monoclonal

Concentration	1µg/ul
Purity	Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C

Description	FACS ; IP ; WB

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SKU	Supplier No.	Size	Your price	Quantity
BHA10922465	Y059133	100 ug	$615		Add to Cart

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