The anti-caspase-9 polyclonal antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9.
Isotype
IgG
Quantity
100 µl
Reactivity
Rat, Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
1µg/ul
Description
Specificity: This Polyclonal antibody is directed against human Caspase-9 protein. The product was purified from seurm by protein A chromatography.Relevance: Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf 3(1,2). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32(2). Activated caspase-9 cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis(3). Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis inducers including TNFβ,TRAIL, anti-CD-95, FADD, and TRADD(4). Caspase-9 is expressed in a variety of human tissues(2,3).
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/1000.Predicted molecular weight: 46 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB