This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Host
Rabbit
Immunogen
peptide from the intermediate residues of human Caspase 3 protein
Quantity
100 µl
Reactivity
Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Concentration
100µg/100ul
Description
Specificity: Caspase 3 antibody detects endogenous levels of Caspase 3 protein. Not tested with other proteins
Format
Liquid
Purity
Peptide affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, ELISA
Description
Recommended dilutions ELISA: 1:80,000 WB: 1/200 - 1/1000 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA