Blood group Lewis alpha-4-fucosyltransferase ; Fucosyltransferase 3 ; Fucosyltransferase III ; FucT-III ; FUT3 ; Galactoside 3(4)-L-fucosyltransferase ; gastric mucin ; leB ; lewis antigen system ; lewis B blood group antigen
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
19p13.3
Clonality
monoclonal
Description
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.
Host
Mouse
Immunogen
Mucin isolated from a human ovarian cyst fluid
Isotype
IgG1 Kappa
Positive Control
Colon
Reactivity
Human, Guinea pig
Recombinant
FALSE
Regulatory
RUO
Swissprot
P21217
Uniprot
169238
Gene Id
2525
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
IF, IHC-F
Description
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., IF ; IHC-F
Dilution
IF
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Blood Group Antigen Lewis B Antibody BHA10201367