Syntheitic peptide at the N-terminus of Beta-Actin. The N-terminus of Beta-Actin is highly conserved with Gamma-Actin.
Isotype
IgG
Quantity
100 µg
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Concentration
100µg/100ul
Description
Specificity: Recognize β-actin from Human and Mouse. Other species not tested yet.
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IP
Description
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:500. IP: 5 mcg/mg lysate., WB ; IP
Dilution
WB
1:500
Reference
Bong-Kyu, K.B et al. "miR‐330‐5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression." FEBS Journal 24:1742-4658 (2015). DOI: 10.1111/febs.13523.
Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J Virol 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. Application: Western Blot.
Horakova, AH et al. "The macrosatellite DXZ4 mediates CTCF-dependent long-range intrachromosomal interactions on the human inactive X chromosome." Hum Mol Genet 21 (20):4367-4377 (2012). DOI: 22791747. Application: Western Blot.
Kim, B.K. et al. "Hairless Up‐Regulates Tgf‐β2 Expression via Down‐Regulation of miR‐31 in the Skin of “Hairpoor”(HrHp) Mice." J. Cell. Physiol :2075–2085 (2015). DOI: 10.1002/jcp.24935.
Kim, BH et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene." J Biol Chem 287 (20):16681-16688 (2012). DOI: 10.1074/jbc.M111.320770. Application: Western Blot.
Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors." Neuron 67:984-96 (2010). PubMed: 20869595.
Reviews of Anti-Beta-Actin Polyclonal Loading Control Antibody