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Home / Antibodies

Anti-ATF6 Antibody

BHA10917604
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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Request More Infos
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Buffer PBS, pH 7.4 with 0.02% Sodium Azide
Concentration 100µg/100ul
Description Specificity: ATF6 antibody detects endogenous levels of ATF6 protein. Not tested with other proteins
Relevance: ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.
Format Liquid
Purity Purified
Storage This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition -20C
Applications WB, ELISA
Description Recommended dilutions Peptide ELISA: 1:20,000 WB: 1/100 - 1/500 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA
Dilution

ELISA1:20,000
WB1:100-1:500
SKU Supplier No. Size Your price Quantity
BHA10917604 Y058063 100 ul $217.50
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