The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene
Host
Rabbit
Immunogen
Synthetic peptide conjugated to KLH
Isotype
IgG
Quantity
100 µl
Reactivity
Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Concentration
100µg/100ul
Description
Specificity: Asparagine synthetase antibody detects endogenous levels of Asparagine synthetase protein. Not tested with other proteinsRelevance: Asparagine synthetase is a housekeeping enzyme responsible for the production of aspargine from aspartate and glutamine. Most mammalian cells express Asparagine synthetase and regulate the level of activity in the response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no Asparagine synthetase activity and rely on the surrounding medium as a source of exogenous asparagines. Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia.
Format
Liquid
Purity
Protein A purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, ELISA
Description
Recommended dilutions Peptide ELISA: 1:20,000 WB: 1/100 - 1/500 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA