APG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: APG12-APG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast APG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Host
Rabbit
Immunogen
A peptide corresponding to the C-terminal of human APG10 protein has been used as the immunogen.
Quantity
100 µl
Reactivity
Rat, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
1µg/ul
Description
Specificity: IHC, WB and ELISA confirmed the specificity for APG10.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
IF, IHC, ICC
Description
A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user., IF ; IHC ; ICC