Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).
Clonality
polyclonal
Description
Rabbit polyclonal to AKT1
Host
Rabbit
Immunogen
Synthetic peptide derived from Human AKT1.
Isotype
IgG
Quantity
100 µl
Reactivity
Human
Recombinant
TRUE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100µg/100ul
Description
Specificity: This Polyclonal antibody is directed against human AKT1 protein. The product was purified from serum by protein A chromatography. Expect reactivity with human Apaf1. Cross-reactivity with AKT1 from other sources has not been determined.Relevance: The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/500~5000Predicted molecular weight: 56 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB