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Home / Antibodies

Anti-AKT1 (Ab-450) Antibody

SKU : BHA10917266

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y021502
Categories	Primary Antibodies
Clonality	polyclonal
Description	The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Host	Rabbit
Immunogen	The antibody was produced against synthesized non-phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
Isotype	IgG
Quantity	100 µg
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	Rabbit IgG in phosphate buffered saline (without Mg²+ and Ca²+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	100µg/100ul
Description	Specificity: AKT1 (Ab-450) Antibody detects endogenous levels of total AKT1 protein.
Format	Liquid
Purity	Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, IP, IHC

Description

WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200, WB ; IP ; IHC

Dilution

WB	1:500-1:1000~
IHC	1:50-1:100~
IF	1:100-1:200

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SKU	Supplier No.	Size	Your price	Quantity
BHA10917266	Y021502	100 ug	$427.50		Add to Cart

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