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Home / Primary Antibodies / Polyclonal Antibodies

Anti-AKAP12 Antibody Picoband®

SKU : BHA21002801

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Mfr.No.	A02303-3, A02303-3-10ug, A02303-3-Biotin, A02303-3-Cy3, A02303-3-Dylight488, A02303-3-Dylight550, A02303-3-Dylight594, A02303-3-FITC, A02303-3-HRP, A02303-3-APC, A02303-3-PE, A02303-3-iFluor647, A02303-3-carrier-free
Alternative Names	Tumor necrosis factor receptor superfamily member 14
Background	A-kinase anchor protein 12, aka AKAP250, is an enzyme that in humans is encoded by the AKAP12 gene. The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinase A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alteative splicing of this gene results in two transcript variants encoding different isoforms.
Clonality	Polyclonal
Cross Reactivity	No cross-reactivity with other proteins.
Description	Boster Bio Anti-AKAP12 Antibody Picoband® catalog # A02303-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Function	Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into C
Host	Rabbit
Immunogen Region	E.coli-derived mouse AKAP12 recombinant protein (Position: E387-H1620).
Isotype	Rabbit IgG
Molecular Weight	300 kDa
Reactivity	Mouse, Rat
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Synonyms	Tumor necrosis factor receptor superfamily member 14;Herpes virus entry mediator A;Herpesvirus entry mediator A;HveA;Tumor necrosis factor receptor-like 2;TR2;CD270;TNFRSF14;HVEA, HVEM;UNQ329/PRO509;
Tissue Specificity	Widely expressed, with the highest expression in lung, spleen and thymus.
Note	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Uniprot	Q9WTQ5
Gene Id	83397
Gene Name	Akap12
Subcellular Location	Membrane ; Single-pass type I membrane protein .

Conjugates	Biotin, Cy3, Dylight488, Dylight550, Dylight594, FITC, HRP, APC, PE, iFluor647, carrier-free

Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Form	Lyophilized
Purification	Immunogen affinity purified.
Storage	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Applications	ELISA, Flow Cytometry, IF, IHC, ICC, WB
Description	Weste blot, 0.1-0.25ug/ml, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 2-5ug/ml, Mouse<br> Immunocytochemistry/Immunofluorescence, 5ug/ml, Mouse<br> Flow Cytometry (Fixed), 1-3ug/1x10<sup>6</sup> cells, Mouse, Rat<br> ELISA, 0.1-0.5ug/ml, -<br>

Reference	1. Gordon, T., Grove, B., Loftus, J. C., O'Toole, T., McMillan, R., Lindstrom, J., Ginsberg, M. H. Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera. J. Clin. Invest. 90: 992-999, 1992.<br /><br />2. Lee, S.-W., Kim, W. J., Choi, Y. K., Song, H. S., Son, M. J., Gelman, I. H., Kim, Y.-J., Kim, K.-W. SSeCKS regulates angiogenesis and tight junction formation in blood-brain barrier. Nature Med. 9: 900-906, 2003.<br /><br />3. Nauert, J. B., Klauck, T. M., Langeberg, L. K., Scott, J. D. Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr. Biol. 7: 52-62, 1997.

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21002801-1	A02303-3	-	100 ug	$370		Add to Cart
BHA21002801-2	A02303-3-10ug	Sample	10 ug	$99		Add to Cart
BHA21002801-3	A02303-3-Biotin	Biotin	100 ug	$570		Add to Cart
BHA21002801-4	A02303-3-Cy3	Cy3	100 ug	$570		Add to Cart
BHA21002801-5	A02303-3-Dylight488	Dylight488	100 ug	$570		Add to Cart
BHA21002801-6	A02303-3-Dylight550	Dylight550	100 ug	$570		Add to Cart
BHA21002801-7	A02303-3-Dylight594	Dylight594	100 ug	$570		Add to Cart
BHA21002801-8	A02303-3-FITC	FITC	100 ug	$570		Add to Cart
BHA21002801-9	A02303-3-HRP	HRP	100 ug	$570		Add to Cart
BHA21002801-10	A02303-3-APC	APC	100 ug	$670		Add to Cart
BHA21002801-11	A02303-3-PE	PE	100 ug	$670		Add to Cart
BHA21002801-12	A02303-3-iFluor647	iFluor647	100 ug	$670		Add to Cart
BHA21002801-13	A02303-3-carrier-free	carrier-free	100 ug	$370		Add to Cart

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