Mitochondrial intermembrane space. Translocated to the nucleus upon induction of apoptosis.
Clonality
polyclonal
Description
Rabbit polyclonal to AIF
Host
Rabbit
Immunogen
Synthetic peptide derived from Human AIF.
Isotype
IgG
Quantity
100 µl
Reactivity
Rat, Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
1µg/ul
Description
Specificity: This Polyclonal antibody is directed against human,Mouse and Rat AIF protein. The product was purified from seurm by protein A chromatography.Relevance: Apoptosis-inducing factor (AIF) is a highly conserved mitochondrial protein with roles in redox-biochemistry and apoptosis.1,2 Apoptosis is a controlled process of cell death necessary for proper physiological development and maintenance. Loss of mitochondrial membrane potential results in the release of several proteins critical to the acceleration of apoptosis.3 When AIF is released from the mitochondrial intermembrane space it migrates to the nucleus to initiate chromatin condensation and DNA cleavage.4 AIF is recognized by immunoblotting at 67 kDa in most tissues and cell lines.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/2000.Predicted molecular weight: 67 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB