Anti-ADAMTS5 Antibody

Mfr.No.	Y058017
Categories	Primary Antibodies
Cellular Localization	Secreted. Associated with the extracellular matrix
Clonality	polyclonal
Description	This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage.
Host	Rabbit
Immunogen	Synthetic peptide conjugated to KLH
Isotype	IgG
Quantity	100 µl
Reactivity	Human
Recombinant	TRUE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	PBS, pH 7.4 with 0.02% Sodium Azide
Concentration	100µg/100ul
Description	Specificity: Not tested with other proteins Relevance: ADAMTS5, also known as Implantin or Aggrecanase 2, is a member of the larger family of ADAM (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin-5 motif) was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis, and potentially a role in embryo implantation. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms.
Format	Liquid
Purity	Purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, ELISA

Description

Recommended dilutions Peptide ELISA: 1:160,000 . WB: 1/500 - 1/1000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA

Dilution

ELISA	1:160,000
WB	1:500-1:1000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10900094	Y058017	100 ul	$217.50		Add to Cart

Anti-ADAMTS5 Antibody

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