Specificity: This affinity purified antibody is directed against human Actin. The product was affinity purified from antiserum by immunoaffinity purification. Cross-reactivity with Actin from other sources has not been determined.Relevance: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
Format
Liquid
Purity
Affinity purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB
Description
Recommended dilutionsWB: 1/1000.Predicted molecular weight: 42 kDa.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user., WB
Dilution
WB
1:1000
Reference
Delay, C et al. "Increased LINGO1 in the cerebellum of essential tremor patients." Mov. Discord : (2014). PubMed: 24531928. Application: Western Blot.
Tremblay, C et al. "Association of Neuropathological Markers in the Parietal Cortex With Antemortem Cognitive Function in Persons With Mild Cognitive Impairment and Alzheimer Disease." Journal of Neuropatholgy & Experimental Neurology 2:70-88 (2017). DOI: 10.1093/jnen/nlw109. Application: Western Blot.