This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinic GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Host
Rabbit
Immunogen
A peptide corresponding to amino acids 278-289 of ACK1 protein.
Isotype
IgG
Quantity
100 µl
Reactivity
Mouse, Human
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Concentration
100µg/100ul
Description
Specificity: Not tested with other proteins
Format
Liquid
Purity
Purified
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, ELISA
Description
Recommended dilutions Peptide ELISA: 1:16,000 WB: 1/500 - 1/1000. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user, WB ; ELISA