Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The alpha-cardiac and alpha-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle alpha-actin and gamma-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The alpha-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells .
Clonality
Polyclonal
Description
Rabbit Anti-alpha-SMA Polyclonal Antibody
Formulation
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, Liquid solution
Host
Rabbit
Immunogen
Synthesized peptide derived from Alpha-SMA at AA range: 171-220
Immunogen Region
Synthesized peptide derived from alpha-SMA at AA range: 171-220
Isotype
Rabbit IgG
Molecular Weight
42 KD
Others
alpha-SMA Polyclonal Antibody detects endogenous levels of alpha-SMA.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity
Human, Mouse, Rat
Tag
alpha-SMA
Note
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:50-1:300), ELISA (1:10000-1:20000). Not yet tested in other applications.
Accession Number
P62736/P68133/P63267/P63261/P68032
Concentration
1 mg/ml, 1 mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Storage Buffer
PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.