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Home / Primary Antibodies / Monoclonal Antibodies

4-Hydroxynonenal Antibody, Clone 12F7 (SMC-511)

SKU : BHA11905502

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StressMarq Biosciences Inc.

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Mfr.No.	SMC-511, SMC-511D, SMC-511D-A390, SMC-511D-A488, SMC-511D-A594, SMC-511D-APC, SMC-511D-BI, SMC-511D-FITC, SMC-511D-HRP, SMC-511D-PCP, SMC-511D-RPE, SMC-511S
Alternative Names	4-Hydroxynonenal Antibody, 4-Hydroxy Nonenal (4-HNE) Antibody, 4-Hydroxy Nonenal Antibody, 4-HNE Antibody, 4-hydroxy Hexenal Antibody, HNE Antibody, 4-hydroxy-2-nonenal Antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-511 was sufficient for detection of 4-Hydroxynonenal in 0.5 ug of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Description	Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG1
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Antigen	4-Hydroxynonenal
Target	4-Hydroxynonenal
Clone No	12F7
Research Area	Cancer, Oxidative Stress, Lipid peroxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, WB, ICC/IF
Description	WB ; ICC ; IF ; ELISA
Dilution	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Peterson, D., Doorn, J. (2004) Free Radic Biol Med. 37(7):937-945.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11905502-1	SMC-511D	Unconjugated	100 ug	$417		Add to Cart
BHA11905502-2	SMC-511D-A390	ATTO 390	100 ug	$472		Add to Cart
BHA11905502-3	SMC-511D-A488	ATTO 488	100 ug	$471		Add to Cart
BHA11905502-4	SMC-511D-A594	ATTO 594	100 ug	$471		Add to Cart
BHA11905502-5	SMC-511D-APC	APC	100 ug	$470		Add to Cart
BHA11905502-6	SMC-511D-BI	Biotin	100 ug	$467		Add to Cart
BHA11905502-7	SMC-511D-FITC	FITC	100 ug	$462		Add to Cart
BHA11905502-8	SMC-511D-HRP	HRP	100 ug	$458		Add to Cart
BHA11905502-9	SMC-511D-PCP	PerCP	100 ug	$470		Add to Cart
BHA11905502-10	SMC-511D-RPE	RPE	100 ug	$468		Add to Cart
BHA11905502-11	SMC-511S	Unconjugated	12 ug	$45		Add to Cart

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