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Home / Primary Antibodies / Monoclonal Antibodies

4-Hydroxy-2-hexenal Antibody, Clone 5C11.1 (SMC-536)

BHA11905844
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Tel
+1 866.986.9598
Email
orders@biohippo.com
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Antigen 4-Hydroxy-2-hexenal
Target 4-Hydroxy-2-hexenal
Clone No 5C11.1
Research Area Cancer, Oxidative Stress, Lipid peroxidation
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
Shipping Temperature Blue Ice or 4°C
Specificity Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Trademark MOLECULAR SIGNATURE
Applications ELISA, WB
Description WB ; ELISA
Dilution WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11905844-1 SMC-536D Unconjugated 100 ug $417
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BHA11905844-2 SMC-536D-A390 ATTO 390 100 ug $472
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BHA11905844-3 SMC-536D-A488 ATTO 488 100 ug $471
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BHA11905844-4 SMC-536D-A594 ATTO 594 100 ug $471
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BHA11905844-5 SMC-536D-APC APC 100 ug $470
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BHA11905844-6 SMC-536D-BI Biotin 100 ug $467
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BHA11905844-7 SMC-536D-FITC FITC 100 ug $462
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BHA11905844-8 SMC-536D-HRP HRP 100 ug $458
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BHA11905844-9 SMC-536D-PCP PerCP 100 ug $470
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BHA11905844-10 SMC-536D-RPE RPE 100 ug $468
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BHA11905844-11 SMC-536S Unconjugated 12 ug $45
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