Polyclonal Antibody to Histone H3 (H3)

Antigen	Histone H3 (H3)
Clone No	D24-5, N47-7, S5-71, D38-6, D8-2, N14, S17-6, S27-1, D8-23, N58-2, C58, N24-5, S8-6

Buffer	0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration	500µg/ml, 1 mg/ml, 0.5 mg/ml, 2mg/ml
Format	Liquid
Purification	Protein A or Protein G purified, Immunogen affinity purified, This product was purified from antiserum by immunoaffinity chromatography., This product was purified from antiserum by ion exchange purified., This product was purified from antiserum by immunoaffinity chromatography, followed by human IgG adsorption. It does combined with human IgG., Cultured cell supernatant, Protein A purified, This product was purified from normal human by octoic acid purified., This product was purified from antiserum by octoic acid purified., This product was purified from antiserum by immunoaffinity chromatography (monoclonal).
Purity	Antigen-specific affinity chromatography followed by Protein A affinity chromatography, >90% by SDS-PAGE, >95% (SDS-PAGE), >90% (SDS-PAGE), >95% by SDS-PAGE
Storage	Avoid repeated freeze/thaw cycles. Store at 4° for frequent use. Aliquot and store at -20° for 12 months.
Storage Buffer	In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide., In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide., In PBS (pH 7.2) with 0.09% NaN and 0.2% BSA., 10mM sodium phosphate, 150mM sodium chloride, pH7.2, In 10 mM Sodium phosphate, 150 mM Sodium chloride, pH 7.4 with 30% glycerol without preservatives., . In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide., 0.01 M PBS, pH7.4, In 10 mM PBS (pH7.4), 30% glycerol without preservatives.
Storage Instructions	Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles., Ship at 4°C, after receipt, store at 4°C in dark for one year. Avoid repeated freeze and thaw cycles., Store at -20°C for long term. And the slight precipitation may occur if store at 2-8°C. Avoid repeated freeze and thaw cycles., Store at 4°C for frequent use, at -20°C for long term. And store the diluted solution at 4°C. Avoid repeated freeze and thaw cycles., Store at -20°C for one year. Avoid repeated freeze and thaw cycles., Ship at 4°C, after receipt, aliquot and store at -20°C for long term. Avoid repeated freeze and thaw cycles., Store at -20°C in dark for one year. And store the diluted solution at 4°C for repeated use. Avoid repeated freeze and thaw cycles., Store at 4°C for frequent use, at -20°C for long term. Avoid repeated freeze and thaw cycles., Store at -20°C. Avoid repeated freeze and thaw cycles., Store at 4°C for frequent use (6 months), at -20°C for long term (one year). Avoid repeated freeze and thaw cycles

Applications	WB, IHC, ICC, IP
Description	Optimal working dilutions must be determined by end user., WB ; IHC ; ICC ; IP
Dilution	WB 0.5-2μg/mL ICC 5-20μg/mL , It is concentrated product, can be diluted with TBST (pH 7.0)., It is concentrated product, can be diluted with TBST (pH 7.0, including 1% BSA)., It is concentrated product, can be diluted with PBS (pH 7.2).

Datasheet

Reviews of Polyclonal Antibody to Histone H3 (H3)