Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. (UniProt)
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A mouse partial recombinant protein corresponding to amino acids A27-N190 was used as the immunogen for the VEGFA antibody.
Isotype
IgG
Predicted Reactivity
Mouse, Rat
Reactivity
Mouse, Rat
Recombinant
No
Antigen
VEGFA
Uniprot
Q00731
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the VEGFA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
ELISA, WB
Dilution
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant mouse protein) (BSA-free format available)