VCP Antibody / Transitional endoplasmic reticulum ATPase

Mfr.No.	R31778
Clonality	Polyclonal
Description	Valosin-containing protein, also called Cdc48, is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+(ATPase associated with various activities) protein family. The gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is also involved in DNA damage response: recruited to double-strand breaks(DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Amino acids 732-760 (RRDHFEEAMRFARRSVSDNDIRKYEMFAQ) were used as the immunogen for the VCP antibody (100% homologous in human, mouse and rat).
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Rat, Mouse, Human
Recombinant	No

Antigen	VCP
Uniprot	P55072

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the VCP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	IF, FACS, IHC-F, IHC-P, WB
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-5ug/ml