Guanine nucleotide exchange factor VAV3 is a protein that in humans is encoded by the VAV3 gene. This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids D507-E702) was used as the immunogen for the VAV3 antibody.
Isotype
Rabbit IgG
Reactivity
Human
Recombinant
No
Antigen
VAV3
Uniprot
Q9UKW4
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the VAV3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
Direct ELISA, IF, WB
Dilution
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml