Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Categories
Primary Antibodies
Cellular Localization
Nucleus, Ytoplasm
Clonality
polyclonal
Description
Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Host
Rabbit
Immunogen
ubiquitin-conjugating enzyme E2F(putative)
Isotype
IgG
Molecular Weight
21 kDa
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Subcellular Location
nucleus,cytoplasm
Synonyms
NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme 2, NEDD8 protein ligase UBE2F, UBE2F
Antigen
UBE2F
Uniprot
Q969M7
Gene Id
140739
Research Area
Epigenetics, Metabolism
Weight
21kDa
Form
liquid
Format
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)