MED20, also called TRFP or TRF-proximal protein, is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
A recombinant human protein corresponding to amino acids M1-R212 was used as the immunogen for the TRFP antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
TRFP
Uniprot
Q9H944
Buffer
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the TRFP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
Direct ELISA, WB
Dilution
Western Blot: 0.5-1ug/ml,Direct ELISA (recombinant human protein): 0.1-0.5ug/ml (BSA-free format available)