TRAM Antibody / TRAM1

Mfr.No.	R31117
Clonality	Polyclonal
Description	By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that the protein influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	An amino acid sequence from the C-terminus of human Translocating chain-associating membrane protein 1 (KFINFQLRRWREHSAFQA) was used as the immunogen for this TRAM antibody (100% homologous in human, mouse and rat).
Isotype	IgG
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Mouse, Rat, Human
Recombinant	No

Antigen	TRAM
Uniprot	Q15629

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the TRAM antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	IHC-P, IHC-F, WB, ICC
Dilution	Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml