T-cell immunoglobulin and mucin domain-containing protein 1 (TIM-1) or Kidney injury molecule 1 (KIM-1), also known as HAVCR1 and HAVCR, is a protein that in humans is encoded by the KIM1 gene. Biochemical, mutational, and cell adhesion analyses confirm that TIM-1 is capable of homophilic interactions. The features identified in murine TIM1 is conserved in human protein. The protein is a receptor for virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse TIM-1, expression was seen after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression during mouse T-cell differentiation leads to production of the Th2-type cytokine IL4, but not the Th1-type cytokine Ifng.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids 289-307 (HPRAEDNIYIIEDRSRGAE-rat) were used as the immunogen for this TIM-1 antibody.
Isotype
IgG
Predicted Reactivity
Human, Rat
Reactivity
Human, Rat
Recombinant
No
Antigen
TIM-1
Uniprot
O54947
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
The lyophilized TIM-1 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
IHC-F, IHC-P, WB
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml