Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Amino acids RRPIAQWHTLQVEEEVDAMLAVKK were used as the immunogen for the SYT1 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Rat, Human, Mouse
Recombinant
No
Subcellular Location
Cytoplasm, secretory vesicle membrane
Antigen
Synaptotagmin 1
Uniprot
P21579
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the SYT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, FACS
Dilution
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells