Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. It is mapped to Xp11.23. This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability.
Host
Rabbit
Immunogen Region
Amino acids DLSIEVEFEYPFRLHQVYFDAPTCR from the human protein were used as the immunogen for the Synaptophysin antibody.
Isotype
Rabbit IgG
Reactivity
Human, Mouse, Rat
Recombinant
No
Antigen
Synaptophysin
Uniprot
P08247
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
IF, IHC-P, WB
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml